Trypsin/Lys-C Mix, Mass Spec Grade from Promega

Product Specs
ItemTrypsin/Lys-C Mix, Mass Spec Grade
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberV5071
Quantity20microg

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Promega

2800 Woods Hollow Rd.

Madison, Wisconsin

United States

Phone: 800-356-9526
Fax: 800-356-1970

Company Profile

Website: http://www.promega.com

(1) Protocol for establishing a protein interactome based on close physical proximity to a target protein within live budding yeastSTAR ProtocolsDecember 1, 2023Janhavi A. Kolhe, Neethu L. Babu, Brian C. FreemanXL-1500 with 365 nm bulbs,Spectro-UV,Cat#1195T76 "Trypsin/Lys-C Mix, Mass Spec Grade",Promega,Cat#V5071 "AssayMAP C18 cartridges, 96/pk",Agilent,Cat#5190-6532 AssayMAP Bravo Protein and Peptide Sample Prep

(2) Unravelling the Proteomic Profiles of Bovine Colostrum and Mature Milk Derived from the First and Second LactationsFoodsNovember 7, 2023Zhen Feng, Yan Shen, Gongjian Fan, Tingting Li, Caie Wu, Yuhui Yeof samples were detected based on the Bradford method [16] and then normalized to 1 mg/mL. Trypsin (V5071, Promega, Madison, WI, USA) was added at a ratio of 50:1 (w/w) and the solution was incubated at 37

(3) Identification of a Novel Acid Sphingomyelinase Activity Associated with Recombinant Human Acid CeramidaseBiomoleculesNovember 6, 2023Xingxuan He, Edward H. Schuchmanfinal concentration of 1 M. Half of the sample was digested overnight with trypsin/LysC (Promega, #V5072) at a ratio of 1:10 (protein/enzyme; w:w) and the other half was digested overnight with Asp-N (Promega,

(4) Functional diversification despite structural congruence in the HipBST toxin-antitoxin system of Legionella pneumophilamBioOctober 31, 2023Jordan D. Lin, Peter J. Stogios, Kento T. Abe, Avril Wang, John MacPherson, Tatiana Skarina, Anne-Claude Gingras, Alexei Savchenko, Alexander W. Ensminger(25°C, 45 min). Samples were digested for 2 h at 25°C with constant agitation with a Trypsin/LysC mix (V5071, Promega) and overnight at 25°C with proteomics-grade porcine trypsin (T6567, Sigma-Aldrich). For both

(5) The protein phosphatase PPKL is a key regulator of daughter parasite development in Toxoplasma gondiimBioOctober 25, 2023Chunlin Yang, Emma H. Doud, Emily Sampson, Gustavo Arrizabalagaphosphoproteomics, 1:70 protease: substrate ratio, mass spectrometry grade, Promega Corporation, Cat No: V5072). Digestions were acidified with trifluoroacetic acid (TFA, 0.5% vol/vol) and desalted on Sep-Pak Vac

(6) Native lamin A/C proteomes and novel partners from heart and skeletal muscle in a mouse chronic inflammation model of human frailtyFrontiers in Cell and Developmental BiologyOctober 23, 2023Fatima D. Elzamzami, Arushi Samal, Adith S. Arun, Tejas Dharmaraj, Neeti R. Prasad, Alex Rendon-Jonguitud, Lauren DeVine, Jeremy D. Walston, Robert N. Cole, Katherine L. WilsonTrypsin/LysC mixture [40 ug proteases in 1.2 mL of 100 mM triethylammonium bicarbonate (TEAB; Promega #V5071) or approximately 3.33 ug protease per sample]. Individual samples (40 ug) were labeled with a unique

(7) Understanding COVID-19 progression with longitudinal peripheral blood mononuclear cell proteomics: Changes in the cellular proteome over timeiScienceOctober 20, 2023Giuseppe Gianini Figueirêdo Leite, Milena Karina Colo Brunialti, Paula M. Peçanha-Pietrobom, Paulo R. Abrão Ferreira, Jaquelina Sonoe Ota-Arakaki, Edecio Cunha-Neto, Bianca Lima Ferreira, Graziella E. Ronsein, Alexandre Keiji Tashima, Reinaldo Salomão34851 DL-Dithiothreitol,Sigma-Aldrich,Cat# D0632 Iodoacetamide,Sigma-Aldrich,Cat# I1149 Trypsin/Lys-C Mix,Promega,Cat# V5071 50% Hydroxylamine for TMT experiments,Thermo Scientific,Cat# 90115 Urea,Sigma-Aldrich,Cat# 51456 Thiourea,Sigma-Aldrich,Cat#

(8) Loss of succinyl-CoA synthetase in mouse forebrain results in hypersuccinylation with perturbed neuronal transcription and metabolismCell ReportsOctober 17, 2023Makayla S. Lancaster, Byungwook Kim, Emma H. Doud, Mason D. Tate, Ahmad D. Sharify, Hongyu Gao, Duojiao Chen, Ed Simpson, Patrick Gillespie, Xiaona Chu, et al.Aldrich,Cat.No.: C4706 Chloroacetamide,Sigma Aldrich,Cat.No.: C0267 Trypsin/Lys-C,Promega,Cat.No.: V5072 NP-40,IBI Scientific,Cat.No.: 9016–45-9 Trifluoroacetic acid,Alfa Aesar,Cat.No.: LO6374 "Tandem Mass

(9) Taste papilla cell differentiation requires the regulation of secretory protein production by ALK3-BMP signaling in the tongue mesenchymeDevelopmentSeptember 15, 2023Mohamed Ishan, Zhonghou Wang, Peng Zhao, Yao Yao, Steven L. Stice, Lance Wells, Yuji Mishina, Hong-Xiang Liuphosphine hydrochloride, alkylated with 13.75 mM of iodoacetamide and digested with trypsin/Lys-C mix (V5071, Promega). The resulting peptides were cleaned up with Acclaim PepMap 100 C18 spin columns (SEM SS18V,

(10) NLRP14 Safeguards Calcium Homeostasis via Regulating the K27 Ubiquitination of Nclx in Oocyte‐to‐Embryo TransitionAdvanced ScienceSeptember 1, 2023Tie‐Gang Meng, Jia‐Ni Guo, Liu Zhu, Yike Yin, Feng Wang, Zhi‐Ming Han, Lei Lei, Xue‐Shan Ma, Yue Xue, Wei Yue, et al.and alkylated with 10 mM iodoacetamide (Sigma, I1149). After digestion by Trypsin/Lys‐c Mix (Promega, V5071) overnight, the peptide solution was desalted using SOLAµ HRP SPE spin plates (Thermo Scientific, 60209‐001).

(11) Protocol for deep proteomic profiling of formalin-fixed paraffin-embedded specimens using a spectral library-free approachSTAR ProtocolsSeptember 1, 2023Rajesh Kumar Soni(NaOH),Sigma-Aldrich,221465-500G Sera-Mag SpeedBeads,Thermo Scientific,4515-2105-050250 Trypsin/Lys-C Mass spec grade,Promega,V5071 "Trifluoroacetic acid, sequencing grade (TFA)",Thermo Fisher Scientific,28904 Ethylacetate,Millipore

(12) Characterization of endoplasmic reticulum-associated degradation in the human fungal pathogen Candida albicansPeerJAugust 25, 2023Ellen M. Doss, Joshua M. Moore, Bryce H. Harman, Emma H. Doud, Eric M. Rubenstein, Douglas A. Bernsteinprotein digestion at 37 °C (1:70 protease:substrate ratio, Mass Spectrometry grade, Promega Corporation, Cat No: V5072.). Peptide purification and labeling: Digestions were acidified with trifluoroacetic acid (TFA, 0.5%

(13) A micro-flow, high-pH, reversed-phase peptide fractionation and collection system for targeted and in-depth proteomics of low-abundance proteins in limiting samplesMethodsXJuly 31, 2023Marta Zurawska, Mark Basik, Adriana Aguilar-Mahecha, Michal Dadlez, Dominik Domanskipurchased from Sigma-Aldrich (Saint Louis, USA). MS-grade trypsin/Lys-C was purchased from Promega (# V5071, Fitchburg, WI). Equipment The following equipment is required: 1st-dimension – High-pH RP-LC micro-flow

(14) Monitoring both extended and tryptic forms of stable isotope-labeled standard peptides provides an internal quality control of proteolytic digestion in targeted mass spectrometry-based assaysMolecular & Cellular ProteomicsJuly 20, 2023Rachel A. Lundeen, Jacob J. Kennedy, Oscar D. Murillo, Richard G. Ivey, Lei Zhao, Regine M. Schoenherr, Andrew N. Hoofnagle, Pei Wang, Jeffrey R. Whiteaker, Amanda G. Paulovichexperiments were obtained from Promega: trypsin (MS sequencing grade, V5111) and trypsin/Lys-C mix (V5072). A spiked internal retention time (iRT) standard set of 11 peptides was obtained from Biognosys (Ki-3002-1). Extended

(15) Deoxyhypusine synthase mutations alter the post-translational modification of eukaryotic initiation factor 5A resulting in impaired human and mouse neural homeostasisHGG AdvJuly 13, 2023Padgett LR, Shinkle MR, Rosario S, Stewart TM, Foley JR, Casero RA Jr, Park MH, Chung WK, Mastracci TL.overnight protein digestion at 37°C (1:70 protease:substrate ratio, mass spectrometry grade, Promega, no. V5072). Digestions were acidified with trifluoroacetic acid (TFA) (0.5% v/v) and desalted on Sep-Pak Vac cartridges

(16) Proteome-wide quantitative analysis of redox cysteine availability in the Drosophila melanogaster eye reveals oxidation of phototransduction machinery during blue light exposure and ageRedox BiologyJuly 1, 2023Sarah C. Stanhope, Tal Brandwine-Shemmer, Hannah R. Blum, Emma H. Doud, Amber Jannasch, Amber L. Mosley, Baruch Minke, Vikki M. Weakeproteins were digested overnight at room temperature with trypsin/LysC (ratio of 1:50, Promega, Cat #V5072). After quenching the digestion with 0.5% TFA, samples were fractionated with a Pierce high pH basic

(17) The protein phosphatase PPKL is a key regulator of daughter parasite development in Toxoplasma gondiibioRxivJune 17, 2023Chunlin Yang, Emma H. Doud, Emily Sampson, Gustavo Arrizabalagaphosphoproteomics, 1:70 protease: substrate ratio, Mass Spectrometry grade, Promega Corporation, Cat No: V5072.) Digestions were acidified with trifluoroacetic acid (TFA, 0.5% v/v) and desalted on Sep-Pak® Vac cartridges

(18) Deep Proteomics Network and Machine Learning Analysis of Human Cerebrospinal Fluid in Japanese Encephalitis Virus InfectionJournal of Proteome ResearchJune 2, 2023Tehmina Bharucha, Bevin Gangadharan, Abhinav Kumar, Ashleigh C. Myall, Nazli Ayhan, Boris Pastorino, Anisone Chanthongthip, Manivanh Vongsouvath, Mayfong Mayxay, Onanong Sengvilaipaseuth, et al.below 1 M. Digestion was performed with trypsin in a ratio of 1:20 m:m protein:trypsin (Promega, P/N V5072 for the pilot study; V5117 for the larger study); first 75% of the total amount of trypsin added and

(19) RNA polymerase II-associated proteins reveal pathways affected in VCP-related amyotrophic lateral sclerosisBrainJune 1, 2023Mahmoud-Reza Rafiee, Sara Rohban, Karen Davey, Jernej Ule, Nicholas M Luscombeneutralized by adding DTT (10 mM) again. The proteins were digested with 300 ng trypsin/LysC-mix (Promega, V5071) for 14–16 h at 37°C. Finally, the peptides were desalted using Ziptips with 0.6 µl C18 resin (Merck).

(20) A druggable copper-signalling pathway that drives inflammationNatureMay 1, 2023Solier S, Müller S, Cañeque T, Versini A, Mansart A, Sindikubwabo F, Baron L, Emam L, Gestraud P, Pantoș GD, et al.reach a final concentration of 1 M urea and digested overnight at 37 °C with Trypsin:Lys-C (Promega, V5071) at a ratio of 1:50. Samples were then loaded onto a homemade C18 StageTips for desalting. Peptides

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